Elimination of Salmonella spp. by hexadecylpyridinium chloride [Polish]

摘要

The aim of this study was to determine the influence of hexadecylpyridinium chloride concentrations on Salmonella spp. in bacteriological media and on turkey carcasses. The average bacteria counts in the control samples not containing hexaadecylpyridinium chloride were: 1.7x108, 2.3x108 and 2.3x108 for S. Anatum, S. Enteritidis and S. Typhimurium, respectively. Hexadecylpyridinium chloride in 1%-concentration agar media completely inhibited the growth of all the Salmonella spp. being studied. The bacterial count decreased by 4 log cycles at a concentration of 0.5%, and at a concentration of 0.25% - by 1 log cycle, compared with the control cycle. However, the bacteria studied grew in the presence of 0.03%, 0.02% and 0.01% of hexadecylpyridinium chloride in the amounts within the same logarithm range. The results obtained after immersing turkey carcass elements in hexadecylpyridinium chloride indicated clearly that detecting Salmonella spp. in the samples was dependent on the inoculum of these bacteria on the poultry carcass surface. When contaminating 101 or 102 colony forming units (cfu) of Salmonella spp. on the surface of a turkey carcass element and immersing it in water solutions of 0.1% and 0.5% hexadecylpyridinium chloride for 15 minutes, no Salmonella spp. were found. After contaminating 103 cfu of Salmonella Enteritidis on the surface of a turkey carcass part and immersing it in a water solution of 0.5% hexadecylpyridinium chloride, the number of samples in which Salmonella spp. were found decreased in comparison with the number of control samples. After contaminating 104 cfu on the surface of a turkey carcass immersed in 0.1% and 1% hexadecylpyridinium chloride no effect on Salmonella spp. detectability was found.