Detection of Flavobacterium psychrophilum in fry of rainbow trout by RT-PCR

摘要

The paper describes the occurence of Flavobacterium psychrophilum as a concurrent pathogen with infectious hematopoietic necrosis virus in rainbow trout fry in Croatia. A molecular technique was applied for F. psychrophilum detection due to the failure of standard bacterial identification on agar plates. As the tissue material had already been used up for RNA extraction and detection of RNA virus, classical, well-described PCR could not be performed. Consequently, one step RT-PCR was adapted for F. psychrophilum identification. This method proved to be a valuable diagnostic tool for F. psychrophilum detection in mixed co-infections with RNA viruses.