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首页> 外文期刊>Medical oncology >Clinical significance of vascular endothelial growth factor and connexin43 for predicting pancreatic cancer clinicopathologic parameters.
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Clinical significance of vascular endothelial growth factor and connexin43 for predicting pancreatic cancer clinicopathologic parameters.

机译：血管内皮生长因子和连接蛋白43预测胰腺癌临床病理参数的临床意义。

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摘要

Vasiformation is essential for the growth and metastasis of tumor. Vascular endothelial growth factor (VEGF) and connexin43 (Cx43) are important regulatory factors of vasiformation. This study aimed to find out the expression features of VEGF and Cx43 and their significance in pancreatic cancer. The expression levels of VEGF and Cx43 protein in the samples, which came from 100 patients of human pancreatic cancer tissues and adjacent normal pancreatic tissues, were examined by using immunohistochemical streptavidin-peroxidase (S-P) method, Western-blotting, and RT-PCR analyses. Compared with adjacent normal pancreatic tissues, RT-PCR showed that the expression of VEGF mRNA was significantly higher in pancreatic cancer tissues (0.788+/-0.290, P<0.01) and Cx43 mRNA was significantly lower in pancreatic cancer tissues (0.403+/-0.204, P<0.01). The expression of VEGF protein was higher in pancreatic cancer tissue (0.745+/-0.254, P<0.01) by Western-blot, and Cx43 protein was obviously lower in pancreatic cancer tissues (0.373+/-0.164, P<0.01). The immunohistochemical S-P method showed as follows: the positive expression rate of VEGF and Cx43 protein was 77 and 48% in pancreatic cancer tissues and 15 and 100% in adjacent normal pancreatic tissues; expressions of VEGF were related to tumor size, TNM stage, and lymph node metastasis (P<0.05); there was a close relation between the expression of Cx43 and histological grades, TNM stage, and lymph node metastasis (P<0.05). This present study suggests that VEGF is overexpressed and the expression of Cx43 is lower in pancreatic cancer. The expression of VEGF and Cx43 is significantly correlated with TNM stage and lymph node metastasis. Furthermore, VEGF and Cx43 may play an important role in the occurrence, development, and metastasis of pancreatic cancer. To examine VEGF and Cx43 may be of value in judging the malignancy degree and the prognosis of pancreatic cancer.
机译：血管形成对肿瘤的生长和转移至关重要。血管内皮生长因子（VEGF）和连接蛋白43（Cx43）是血管形成的重要调控因子。这项研究旨在找出VEGF和Cx43的表达特征及其在胰腺癌中的意义。用免疫组织化学链霉亲和素过氧化物酶（SP）法，Western-blotting和RT-PCR分析检测了100例人类胰腺癌组织和邻近正常胰腺组织的样本中VEGF和Cx43蛋白的表达水平。 。与邻近的正常胰腺组织相比，RT-PCR显示，VEGF mRNA在胰腺癌组织中的表达明显较高（0.788 +/- 0.290，P <0.01），而Cx43 mRNA在胰腺癌组织中的表达则较低（0.403 +/- 0.204，P <0.01）。 Western blot检测胰腺癌组织中VEGF蛋白的表达较高（0.745 +/- 0.254，P <0.01），胰腺癌组织中Cx43蛋白的表达明显较低（0.373 +/- 0.164，P <0.01）。免疫组化S-P法显示：胰腺癌组织中VEGF和Cx43蛋白的阳性表达率分别为77％和48％，邻近正常胰腺组织中的VEGF和Cx43蛋白的阳性表达率分别为15％和100％。 VEGF的表达与肿瘤大小，TNM分期和淋巴结转移有关（P <0.05）; Cx43的表达与组织学分级，TNM分期，淋巴结转移密切相关（P <0.05）。本研究提示胰腺癌中VEGF过度表达，而Cx43的表达则较低。 VEGF和Cx43的表达与TNM分期和淋巴结转移密切相关。此外，VEGF和Cx43可能在胰腺癌的发生，发展和转移中起重要作用。检测VEGF和Cx43可能在判断胰腺癌的恶性程度和预后方面具有重要价值。

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