Rapid extraction and analysis of volatile organic compounds of Iranian feverfew(Tanacetum parthenium)using headspace solvent microextraction(HSME),and gas chromatography/mass spectrometry

摘要

In the present study,we have utilized headspace solvent microextraction(HSME)as a modern and rapid sample preparation method for the isolation and preconcentration of the volatile organic compounds(VOCs)from the headspace of a plant into a microdrop.The extraction was done by suspending a microliter drop of the solvent from the tip of a microsyringe to the headspace of dried and powdered feverfew flowers(Tanacetum parthenium)in a sealed vial for a preset extraction time.Then,the microdrop was retracted back into the microsyringe and injected directly into a GC injection port.In this study,the identification of the VOCs extracted by HSME was confirmed according to their retention indices and mass spectra(EI,70 eV)and quantitative analysis was performed by GC-FID.Parameters such as the nature of the extracting solvent,particle size of the sample,temperatures of the microdrop and sample,volumes of the microdrop and sample and the extraction time were studied and optimized and the method performance was evaluated.The optimized conditions were:sample particle size,0.65 mm;sample volume,8 ml(in a 15 ml vial);sample temperature,50 deg C;microsyringe needle temperature,25 deg C(room temperature);microdrop volume,3 mul and extraction time,8 min.Finally,under optimum working conditions,the percentage of the VOCs extracted from the headspace and the relative standard deviation of the extraction for each compound based on seven replicated analyses were evaluated.