Preparation of core-shell-type poly(ethylene glycol) grafted polystyrene resins and their characteristics in solid-phase peptide synthesis

摘要

By suspension polymerization using styrene, divinylbenzene, and synthetic poly(ethylene glycol) (PEG) macromers, we have prepared core-shell-type resins that have a highly crosslinked polystyrene core and a PEG shell. The PEG macromonomer, which has a vinyl group and an amino group at each terminal, plays a role as a stabilizer in the suspension polymerization system. All the resins were of the bead type witha diameter of 38-150 mum. The loading capacities of the amino groups were 0.1-0.2 mmol(.)(g(.)resin)(-1) and the thickness of the PEG shell 2-4 mum, which was verified by cross-sectioned views of the fluorescamine-coupled beads. During coupling of amino acids in solid-phase peptide synthesis, the resin showed better yields than TentaGel resin, especially during the first coupling step. In the enzymatic cleavage reaction, the resin-bound peptides were shaved about 5-10 times more than TentaGel resin. [References: 26]