Preparation of Photoreactive Azidophenyl Hyaluronic Acid Derivative: Protein Immobilization for Medical Applications

摘要

Photoreactive azidophenyl hyaluronic acid (Az-HA) derivative was synthesized to immobilize proteins such as epidermal growth factor (EGF) and bone morphogenetic protein (BMP). The photosensitizing group, 4-azidoaniline, was introduced to the carboxyl group of hyaluronic acid by a water soluble carbodiimide, l-ethyl-3-(3-dimethylamino-propyl)carbodiimide hydrochloride (EDC). To characterize the synthesized Az-HA, Fourier transform infrared (FTTR), proton nuclear magnetic resonance ('H NMR) and ultraviolet/visible (UV/vis) absorbance spectroscopy were performed. We demonstrated protein immobilization by using fluorescein isothiocyanate conjugated albumin (FITC-BSA) on Az-HA derivative surfaces that were patterned with micro scale. Cytotoxicity and relative cell proliferation tests of the Az-HA derivative were performed on an MG-63 human osteoblast cell line for biocompatibility.