Determination of Cr(III) in urine, blood serum and hair using flow injection chemiluminescence analysis

摘要

A flow injection (FI) chemiluminescence method for the determination of Cr(III) in blood serum, urine and hair samples is reported. It is based on the chromium-catalyzed light emission from the luminol oxidation by hydrogen peroxide. The apparatus consists of an FI system with a flow cell formed by a coiled transparent tube suitable for chemiluminescence detection. The specificity of the method is achieved in presence of EDTA. The detection limit under optimum conditions is 0.01 mu g L-1 of Cr(III). Precision and accuracy were evaluated by determining Cr(III) concentrations in urine standards from the National Institute of Standard and Technology (NIST). [References: 16]