A review of the DNA standard reference materials developed by the National Institute of Standards and Technology

摘要

The Standard Reference Materials Program at. the US National Institute of Standards and Technology (NIST) has three human DNA standard reference materi-als (SRM 2390, SRM 2391a, and SRM 2392) currently availablel [1, 2]. Both the DNA profiling SRM 2390 and the polymerase chain reaction (PCR)-based DNA profil- ing SRM 2391 a are intended for use in forensic and pater-nity identifications, for instructional law enforcement, or for non-clinical research purposes and are not intended for clinical diagnostics. The mitochondrial DNA (mtDNA} SRM 2392 is to provide standardization and quality control when performing PCR and sequencing any segment. or the entire 16,569 base pairs that comprise human mito-chondrial DNA. SRM 2392 is designed for use by the forensic, medical, and toxicological communities for hu- man identification, disease diagnosis or mutation detectipn.