Flow injection-solid phase spectrofluorimetric determination of pyridoxine in presence of group B-vitamins

摘要

A flow-through optosensor has been prepared for the sensitive and selective determination of pyridoxine (vitamin B_6) in aqueous solutions. The sensor was developed in conjunction with a monochannel flow-in-jection analysis system with fluorimetric detection using Sephadex SP-C25 resin as an active sorbent substrate. This method of determination is carried out without any derivatization. The wavelengths of excitation and emission were 295 and 385 nm, respectively. When a HCl (10~(-3) mol L~(-1))/NaCl (3 * 10~(-2) mol L~(-1)) solution is used as carrier solution, the sensor responds linearly in the measuring range of 5-200, 10-400 and 50-1800 ng mL~(-1) with detection limits of 0.33, 0.67, and 5.70 ng mL~(-1) for 2000, 1000 and 200 #mu#L of sample volume, respectively. The relative standard deviation for ten independent determinations is less than 0.75% for 0.2 and 1.0 mL of sample volumes used, and 1.31% for 2.0 mL of sample volume used. the method was satisfactorily applied to the determination of vitamin B_6 in pharmaceutical preparations.