MICROSATELLITE AND ISOZYME MARKERS FOR SEED SOURCE IDENTIFICATION IN SILVER FIR

摘要

The present paper reports on the genetic differentiation of single trees in silver fir (Abies alba Mill.) using a combined approach of isozyme gene markers with cpDNA-microsatellite markers. The question was whether it is possible to distinguish single trees by analysing a sample of their seeds For this purpose needles or buds and seed samples from 20 silver fir individuals of a seed source population were analysed at 11 isozyme gene loci and three chloroplast microsatellite loci. The results of theinvestigation showed that neither by using only isozyme gene markers nor by using only cpDNA-microsatellites was it possible to distinguish all trees genetically However, the combined use of both marker types made the differentiation of all analysed silver firs possible. Moreover, the probabilities of identity (P_(ID)) calculated for both marker types exhibited lower values than the P_(ID) calculated for each single marker system. The use of both markers can be helpful for controlling forest reproductive material and seed source-identification.