首页> 外文期刊>Forensic science international >New alleles and mutational events in D12S391 and D8S1132: sequence data from an eastern German population.
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New alleles and mutational events in D12S391 and D8S1132: sequence data from an eastern German population.

机译:D12S391和D8S1132中的新等位基因和突变事件:来自德国东部人群的序列数据。

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摘要

To investigate the DNA mutation rate and pattern in the hypervariable short tandem repeat (STR) locus D12S391 and in the locus D8S1132, samples from an eastern German population (Dresden area) were analysed. A duplex PCR was applied, using short amplification products for D12S391 (129-177bp) and a modified reverse primer for D8S1132 (127-182bp). The sequences of some rare and new variant alleles are described. At the locus D12S391, 13 regular and six incomplete alleles with different lengths were found, exhibiting several sequence structures. Two isolated father/child mismatches were observed in a total of 648 meioses. Novel alleles 13.1, 14.1 and 27 were discovered at the locus D8S1132. Three parent/child mismatches were found in a total of 672 meioses.
机译:为了研究高变短串联重复序列(STR)基因座D12S391和基因座D8S1132中的DNA突变率和模式,分析了德国东部人口(德累斯顿地区)的样本。使用D12S391(129-177bp)的短扩增产物和D8S1132(127-182bp)的修饰反向引物进行双工PCR。描述了一些罕见和新的变异等位基因的序列。在基因座D12S391,发现了13个具有不同长度的规则等位基因和6个不等位基因,表现出几种序列结构。在总共648个中子中观察到两个孤立的父子不匹配。在位点D8S1132发现了新的等位基因13.1、14.1和27。在总共672个中签中发现了3个父母/孩子不匹配。

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