Simultaneous Determination of 16 Organic Acids in Food by Online Enrichment Ion Chromatography-Mass Spectrometry

摘要

A novel analytical method for simultaneous determination of 16 organic acids in foodstuff using online enrichment ion chromatography-mass spectrometry (IC-MS) was developed. The online enrichment and separation of the organic acids were performed using ion chromatography with a homemade enrichment column and a homemade separation column. The qualitative and quantitative analyses of the organic acids were performed using mass spectrometry in the selected ion monitoring (SIM) mode and atmospheric pressure chemical ionization (APCI) source in a negative mode. Two hundred-microliter samples were injected for analysis using an online enrichment time of 3 min. Sodium hydroxide solution (NaOH) was used for the gradient elution system. The two columns made it possible to have a low limit of detection since it has good enriching and separating capabilities. Sixteen kinds of organic acids were separated completely within 30 min. All the curves showed good linearity (R (2) a parts per thousand yenaEuro parts per thousand 0.9949) within the test ranges. The limits of detection (LODs) were between 0.01 and 0.22 mg/L, and the average recoveries were between 80.5 and 109.8 %. The relative standard deviations (RSDs) were less than 8.02 %. The results indicated that this method was simple, rapid, sensitive, and accurate for the determination of organic acids in various food products.