Preparation and Application of Chitosan-Grafted Multiwalled Carbon Nanotubes in Matrix Solid-Phase Dispersion Extraction for Determination of Trace Acrylamide in Foods Through High-Performance Liquid Chromatography

摘要

In this study, chitosan-grafted multiwalled carbon nanotubes were synthesized and characterized using Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, thermogravimetric analysis, and static and kinetic adsorption experiments. The results showed that the resulting material had good adsorption abilities and rapid dynamic properties for acrylamide. This material was used as a sorbent for development of matrix solid-phase dispersion extraction coupled with high-performance liquid chromatography for the determination of trace acrylamide in foods. Under the optimal conditions, the limit of detection, based on three times the signal to noise ratio of the baseline near the analyte peak, was 1.5 mu g/L, and the relative standard deviation for five replicate extractions of 50 mu g/L acrylamide was 4.3 %. Blank potato and flour samples spiked with acrylamide at different levels, namely, 2.25, 4.50, and 6.75 mu g/kg, were extracted and determined by the developed method, with good recoveries ranging from 85.3 to 94.6 %. This method was used for quantitative analysis of acrylamide in different food samples, including twisted cruller, potato chip, and toast samples. Compared with other methods, this method for the extraction and determination of trace acrylamide in food samples is rapid and simple.