Protective effect of fish oil on 4-Nitroquinoline-1-Oxide (4NQO) induced oral carcinogenesis in rats

摘要

The present investigation was carried out to study the chemo-preventive potential of fish oil against oxidative stress in 4-nitroquinoline-1-oxide (4NQO) induced oral carcinogenesis by measuring the levels of lipid peroxidation markers, antioxidants, and various serum enzyme activities. Fish oil was administered for 15 days, and changes in the levels of lipid peroxidation markers viz. malonaldehyde (MDA) and conjugated dienes (CD) and antioxidants like superoxide dismutase, catalase, reduced glutathione, glutathione-S-transferase and glutathione peroxidase were evaluated. The serum enzyme activities like alanine transaminase, aspartate transaminase, lactate dehydrogenase, creatine phosphokinase and alkaline phosphatase were also examined in normal and 4NQO-administered rats. A significant increase in the activities of antioxidants and a decrease in the formation of MDA and conjugated dienes were observed in oral tissues on treatment with fish oil. The administration of fish oil also significantly reduced the serum enzyme activities. The antioxidant effect of fish oil might be due to the incorporation of eicosapentaenoic acid which is the major component of fish oil, into the membrane phospholipids of oral tissue, thus protecting the cells from the oxidative stress induced by 4NQO.