Blastocyst rate and live births from vitrification and slow-cooled two-cell mouse embryos.

摘要

OBJECTIVE: The purpose of this study was to develop a closed vitrification system, compare vitrification to a slow-cooled cryopreservation method, and compare the pup rate between both methods using two-cell mouse embryos. DESIGN: Randomized, prospective animal study. SETTING: Hospital-based IVF practice. ANIMAL(S): B6C3F1 mouse embryos. INTERVENTION(S): Two-cell mouse embryos were cryopreserved using a slow-cooled or vitrification method and then thawed at a later date. The embryos were cultured and transferred to recipient females. MAIN OUTCOME MEASURE(S): Embryos were observed for blastocyst rate and pups were observed for phenotypic anomalies and weighed at 30, 60, and 90 days after birth. RESULT(S): Neither the blastocyst rate, pup rate, nor pup weights were significantly different when the two cryopreservation methods were compared. CONCLUSION(S): Because there were no differences in blastocyst rates, pup rates, or pup weights, we plan to further investigate the potential effects of vitrificationon genotypic damage via the Comet Assay.