Direct, Noncovalent Coating of a Gold Surface with Polymeric Self-Assembled Monolayers

摘要

The spatio-selective immobilization of biomolecules, such as DNAs, antibodies, or aptamers, onto a solid surface is required for the development of bioanalytical and biomedical devices that interface the immobilized probe with the target biospecifically. Strategies for non-covalent and covalent immobilization have been developed, exemplified by biotin-streptavidin or N-nitrilotriacetic acid (NTA)-histidine tag interactions for non-covalent linking and N-hydroxysuccinimide (NHS)-mediated amide coupling for covalent coupling. In addition to the surface-immobilized bioprobes, the surface should be non-biofouling (i.e., preventing or at least minimizing the non-specific adsorption of proteins and other molecules and adherence of cells) for maximized biospecific recognition between the probe and the target and multiplexed detection of the analytes based on rnicroarrays/patterns. In this respect, a method should be developed for providing a surface of interest with three orthogonal properties: functionalizable, non-biofouling, and surface-anchorable ones. We suggested the formation of polymeric self-assembled monolayers (pSAMs) with a random copolymer having these three properties.