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Design of carbohydrate multiarrays

机译:碳水化合物多阵列设计

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摘要

Recently, microarray technology has increasingly been widely applied in glycobiology. This technology has rather evident potential advantages: unlimited number of carbohydrate ligands coated onto one small sized chip, enormously low consumption of both carbohydrate ligands and carbohydrate-binding proteins to be tested, etc. Literature data demonstrate that three approaches are used for glycoarray design. The first one is based on the physical adsorption of glycomolecules on a surface (as in a common ELISA), the second one-on covalent immobilization, and the third one-on a streptavidin-biotin system. In all of the described methods, carbohydrate ligands were placed on chips as a 2D monolayer and high sensitivity was achieved due to fluorescent detection. Notably, a tendency of stepping from model chips toward real multiarrays, where the number of carbohydrate ligands can be up to two hundred, has been observed the last 2 years, this already producing a number of interesting findings when studying carbohydrate-binding proteins.
机译:最近,微阵列技术已越来越广泛地应用于糖生物学。该技术具有相当明显的潜在优势:在一个小芯片上包被的碳水化合物配体的数量不受限制,要测试的碳水化合物配体和碳水化合物结合蛋白的消耗极低,等等。文献数据表明,三种方法可用于糖阵列设计。第一个是基于糖分子在表面上的物理吸附(如在普通ELISA中一样),第二个是基于一对一的共价固定,第三个是基于链霉亲和素-生物素系统。在所有描述的方法中,将碳水化合物配体作为2D单层放置在芯片上,并且由于荧光检测而获得了高灵敏度。值得注意的是,在过去的两年中,已经观察到从模型芯片向真正的多阵列迈进的趋势,其中碳水化合物配体的数量可以高达200个,这在研究碳水化合物结合蛋白时已经产生了许多有趣的发现。

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