Freezing and desiccation tolerance in the moss Physcomitrella patens: An in situ Fourier transform infrared spectroscopic study




In situ Fourier transform infrared spectroscopy (FTIR) was used in order to obtain more insights in the underlying protective mechanisms upon freezing and drying of ABA-treated tissues of the moss Physcomitrella patens. The effects of different treatments on the membrane phase behaviour, glassy state, and overall protein secondary structure were studied. We found that growth on ABA resulted in the accumulation of sucrose: up to 22% of the tissue on a dry weight basis, compared to only 3.7% in non-ABA-treated tissues. Sucrose functions as a protectant during freezing and drying, but accumulation of sucrose alone is not sufficient for survival. ABA-treated tissue survives a freeze-thaw cycle down to -80 degrees C only after addition of an additional cryoprotectant (DMSO). Survival correlates with preservation of membrane phase behaviour. We found that ABA-treated P. patens can survive slow but not rapid drying down to water contents as low as 0.02 g H2O per g DW Rapidly and slowly dried ABA-treated tissues were found to have similar sugar compositions and glass transition temperatures. The average strength of hydrogen bonding in the cytoplasmic glassy matrix, however, was found to be increased upon slow drying. In addition, slowly dried tissues were found to have a higher relative proportion of a-helical structures compared to rapidly dried tissues. (c) 2006 Elsevier B.V All rights reserved.
机译:使用原位傅里叶变换红外光谱法(FTIR),以便在冷冻和干燥经过ABA处理的苔藓小立碗藓组织后,获得有关潜在保护机制的更多见解。研究了不同处理对膜相行为,玻璃态和整体蛋白质二级结构的影响。我们发现,ABA上的生长导致蔗糖的积累:以干重计最多占组织的22%,而未经ABA处理的组织中只有3.7%。蔗糖在冷冻和干燥过程中起着保护剂的作用,但是仅蔗糖的积累不足以存活。仅在添加其他防冻剂(DMSO)后,经过ABA处理的组织才能在低至-80摄氏度的温度下保持冻融循环。存活与膜相行为的保存相关。我们发现,ABA处理的P. patens可以慢速生存,但不能快速干燥至低至0.02 g H2O / g DW的水含量。快速和缓慢干燥的ABA处理的组织具有相似的糖组成和玻璃化转变温度。然而,发现细胞质玻璃状基质中氢键的平均强度在缓慢干燥后会增加。另外,发现与速干组织相比,速干组织具有较高的α-螺旋结构相对比例。 (c)2006 Elsevier B.V保留所有权利。


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