Investigation of vascular invasion and genetic polymorphisms of DPD IVS14+1 G>A, UGT1A1 3156 G>A, and UGT1A1 28 tandem repeats in colorectal cancer patients in Taiwan

摘要

In the present study, multiple chemotherapeutic agent-related genetic polymorphisms, including dihydropyrimidine dehydrogenase (DPD) IVS14+1 G>A, UGT1A1 3156 G>A, and UDP-glucuronosyltransferase (UGT)1A1 28 tandem repeats, were analyzed in patients with colorectal cancer and studied in correlation with the clinical features of those patients. The genotypes from 273 patients with stages I-IV colorectal cancer who underwent operations were determined by means of polymerase chain reaction-restriction fragment length polymorphism. The results showed that the genotype distribution of DPD GG, UGT1A1 3156 GG, and UGT1A1 28 tandem repeats 5/6 or 6/6 in Taiwanese subjects were 98.4%, 82.2%, and 80.6%, respectively. After analysis of the relationship between the genotypes and clinicopathological data of the patients, a significant correlation was observed between vascular invasion in patients with genetic polymorphisms of. DPDGG, UGT1A13156GG, and UGT1A1 28 repeat 5/6 or 6/6 (OR: 2.236, p = 0.015). There was a statistical correlation between vascular invasion and tumor invasion, lymph node metastasis, cancer stage, differentiation, perineural invasion, and survival (all p < 0.05). The results of the present study highly suggest that DPDGG, UGT1A13156GG, and UGT1A1 28 repeat 5/6 or 6/6 genotypes and vascular invasion could be prognostic factors for Taiwanese patients with colorectal cancer.