Synthetic Attenuated Virus Engineering (SAVE) - A Novel Strategy to Generate Viral Vaccine Candidates

摘要

A Novel Strategy to Generate Viral Vaccine Candidates Advances in synthetic biology pave the way for the creation of new vaccine candidates for a potentially wide range of viruses. The viruses are attenuated by means of recoding the sequences of portions of the genome such that they are codon pair deoptimized but still encode the wildtype amino acid sequence. The recoded sequences that contain hundreds of nucleotide changes compared to the wildtype, are generated by chemical synthesis. Thus far, results are very promising for poliovirus and influenza virus. Vaccinations containing live attenuated viruses have provided relief from numerous fatal diseases in the past, and continue to protect countless people today. In contrast to inactivated vaccines, live attenuated vaccines have the benefit of a more efficient cellular and humoral immune response with a low, sometimes single dose.