Reproducible Cell Assays: Consistency of Cryopreservation is a Necessity

摘要

Cell Cryopreservation is a critical component of cell culture work. The cells which survive the thermodynamic journey from the warm temperature of the incubator to the -196 deg C environment of the liquid nitrogen storage tank are free from the influences of time. This capability provides the cell culturist with a means of taking a snapshot of the culture at a given time in its history. In summary, cryopreservation of cultured cells is a proven and essential process. The preservation of samples of PBMCs, stem cells, patient cells, cell lines and other investigative cell material is of compromised value if a the method of cryopreservation imposes variable and unpredictable influences on the constituents of the emerging cell population. Precision-engineered insulation alcohol-free cell freezing containers such as CoolCell represent standardizable means of providing reproducible cell freezing profiles (figures 1 and 2). These devices can contribute greatly in assuring that valuable experimental assets are not only preserved, but provide consistent and meaningful results.