Cryopreservation carrier of animal cells, cryopreservation and methods for cryopreservation bio device using the same

摘要

Cryopreservation of animal cells that can fix the cells at high density and to reduce the damage that animal cells undergo cryopreservation during operation of the animal cells, also improves the viable cell density and viability of animal cells of frozen-thawed to provide a carrier, and a method for cryopreservation cryopreservation bio device. And a curved surface portion on at least a part of the inner wall is provided with a seat opening of the substantially same shape is a plurality of drilled at a constant pitch, forming the opening, cryopreservation carrier, frozen by loading, It is equal to or greater than the animal cells viable cell density, frozen-thawed by suspension method and / or survival of animal cells where you unzipped. The maximum length of the opening is a 0.5μm~40μm curvature radius 10μm~120μm, of the curved surface. The sheet is a mesh-like body. Cryopreservation bio device, and an animal cell that has been carried on the curved portion with the cryopreservation carrier. Cryopreservation method includes the step of cryopreservation of the above cryopreservation bio device.