Enhancing Performance in Cell Culture: Hydrolysates from Plants for Medium Supplementation

摘要

Back in the 1950s, H. Eagle established that a mixture of amino acids, vitamins, cofactors, carbohydrates, and salts are necessary to support in vitro cell growth. Now, however, it's well known that additional growth factors, hormones, and other biochemical compounds are required to optimally control and regulate cell growth. Traditionally, such supplementation has been achieved by adding animal serum (e.g., fetal bovine serum or FBS) at a concentration of 5-20%.