Effect of Detergent Residues in Goat's Milk on The Response of Screening Methods for Antibiotic Detection

摘要

This study aimed to asses the effect of the presence of residues deriving from detergents used to clean and disinfect milking machines and milk storage tanks on the response of two screening methods to detect antibiotics in goat's milk. Antibiotic-free milk samples spiked with different commercial detergents (5 acids, 5 alkalis and 5 domestic dishwashing liquids) and 2 disinfectants at different concentrations were used. Spiked milk samples were analysed by a microbiological method (Eclipse 100) andby a protein receptors binding test (Twinsensor B/T). Acid detergents and disinfectant products did not affect the response of the methods employed as the obtained results were negative at the tested concentrations. Alkaline detergents and domestic dishwashing liquids gave positive results when concentrations were 4-6 ml/l depending on the product and method employed. These relatively high concentrations are very difficult to encounter if good practices for cleaning milking machines and milk storage tanks on farms are implemented.