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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice.
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Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice.

机译:使用种间重组同基因小鼠对生育力特征进行的百日龄范围一步式制图。

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摘要

In mammals, male fertility is a quantitative feature determined by numerous genes. Until now, several wide chromosomal regions involved in fertility have been defined by genetic mapping approaches; unfortunately, the underlying genes are very difficult to identify. Here, 53 interspecific recombinant congenic mouse strains (IRCSs) bearing 1-2% SEG/Pas (Mus spretus) genomic fragments disseminated in a C57Bl/6J (Mus domesticus) background were used to systematically analyze male fertility parameters. One of the most prominent advantages of this model is the possibility of analyzing stable phenotypes in living animals. Here, we demonstrate the possibility in one-step fine mapping for several fertility traits. Focusing on strains harboring a unique spretus fragment, we could unambiguously localize two testis and one prostate weight-regulating QTL (Ltw1, Ltw2, and Lpw1), four QTL controlling the sperm nucleus shape (Sh1, Sh2, Sh3, and Sh4), and one QTL influencing sperm survival (Dss1). In several cases, thespretus DNA fragment was small enough to propose sound candidates. For instance, Spata1, Capza, and Tuba7 are very strong candidates for influencing the shape of the sperm head. Identifying new genes implied in mammalian fertility pathways is a necessary prerequisite for clarifying their molecular grounds and for proposing diagnostic tools for masculine infertilities.
机译:在哺乳动物中,雄性育性是由众多基因决定的定量特征。到目前为止,已经通过遗传作图方法确定了涉及生育力的几个宽泛的染色体区域。不幸的是,基础基因很难鉴定。在这里,使用53种具有1-2%SEG / Pas(Mus spretus)基因组片段的C105Bl / 6J(Mus domesticus)背景传播的种间重组同基因小鼠品系(IRCSs)系统分析了雄性育性参数。该模型最突出的优点之一是可以分析活体动物的稳定表型。在这里,我们证明了一步法精细绘制几种生育性状的可能性。着眼于具有独特的裂殖片片段的菌株,我们可以明确地定位两个睾丸和一个调节前列腺重量的QTL(Ltw1,Ltw2和Lpw1),四个控制精子核形状的QTL(Sh1,Sh2,Sh3和Sh4),以及一个影响精子存活的QTL(Dss1)。在某些情况下,唇齿动物的DNA片段足够小,可以提出合理的候选方案。例如,Spata1,Capza和Tuba7是影响精子头部形状的非常有力的候选者。鉴定哺乳动物生育力途径中隐含的新基因是阐明其分子基础和提出男性不育症诊断工具的必要先决条件。

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