Expression levels of IL-17 and TNF-alpha in degenerated lumbar intervertebral discs and their correlation

摘要

The present study aimed to investigate the expression and roles of interleukin (IL)-17 and tumor necrosis factor (TNF)-alpha in intervertebral disc degeneration (IDD) and to identify the association between the effects of IL-17 and TNF-alpha in IDD. This may increase understanding of the pathogenic mechanism underlying IDD, and aid the development of alternative therapies. The experimental group consisted of 40 samples of nucleus pulposus tissue obtained from the intervertebral discs (IVDs) of patients with IDD by surgical intervention, and was further divided into an annulus fibrosus disrupted group, comprising 18 patients in which the external annulus was ruptured, and an annulus fibrosus intact group comprising 22 patients. The control group consisted of 20 samples of nucleus pulposus tissue from the IVDs of patients with traumatic lumbar disc fractures. The mRNA and protein expression levels of IL-17 and TNF-alpha in the 50 tissue samples were detected by semi-quantitative reverse transcription polymerase chain reaction and immunohistochemical staining, respectively, and the results were statistically analyzed. The IL-17 and TNF-alpha protein and mRNA expression levels in the annulus fibrosus disrupted and annulus fibrosus intact groups were both higher compared with those in the control group. In addition, the expression levels of IL-17 and TNF-alpha in the annulus fibrosus disrupted group were significantly higher compared with those in the annulus fibrosus intact group (P<0.01). A positive correlation was identified between the mRNA and protein expression levels of IL-17 and TNF-alpha in the experimental group (r=0.957, P<0.01). IL-17 and TNF-alpha may therefore be involved in the progression of human IDD, and may have synergistic effects in the development of IDD.