Poly(phenylene oxide) films modified with allylamine plasma as a support for invertase immobilization

摘要

The paper describes a method for preparation of polymer support suitable for covalent invertase immobilization. Modification of poly(phenylene oxide) films by plasma polymerization of allylamine has been applied to introduce amine functionality on the polymer surface. It has been observed that the polymer surface became covered in plasma by a loosely fixed, moderately hydrophilic layer that should be removed before the immobilization process. The chemical character of the stable sub-layer has been related to several modification parameters: geometry of reactor, mode of plasma action and composition of gaseous mixture. Methods for determination of surface concentration of amine groups have also been presented and discussed from the immobilization point of view. (c) 2006 Elsevier Ltd. All rights reserved.