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Unstable L-Form ofiProteus/iimirabilis/iInduced by Fosf omycin

机译:Fosf omycin 诱导的iProteus/iimirabilis/i不稳定 L 型

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An unstable L-form of Proteus mirabilis was induced on solid medium by sensitivity test discs (200 fig of fosfomycin + 20 μg of glucose-6-phosphate). The L-col-onies were subcultured on agar containing the antibiotic at a concentration of 60 μg/ml. On antibiotic-free medium, all the cells reverted to the bacterial phase. On antibiotic-containing agar, the reversion took place as well although at a much lower frequency. Parents and revertants differed in glucose metabolism while they reacted identically in H2S, indole, and urea test
机译:通过灵敏度测试盘(200 fig fosfomycin + 20 μg glucose-6-phosphate)在固体培养基上诱导不稳定的 L 型奇异变形杆菌。将L-col-onies在含有抗生素的琼脂上以60μg/ ml的浓度传代培养。在无抗生素培养基上,所有细胞都恢复到细菌期。在含抗生素的琼脂上,尽管频率要低得多,但还发生了逆转。父母和恢复者在葡萄糖代谢方面存在差异,而他们在 H2S、吲哚和尿素试验中的反应相同

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