Effects of quercetin on proliferation, apoptosis, adhesion and migration, and invasion of HeLa cells.

摘要

OBJECTIVE: This study sought to explore the effects of quercetin on proliferation, apoptosis, adhesion and migration and invasion of HeLa cells. METHODS: Proliferation and apoptosis of quercetin treated HeLa cells were measured by MTT and Annexin V-FITC/PI; adhesion and migration and invasion of quercetin treated HeLa cells were measured by adhesion assay and wound healing assay and the Transwell chamber method, respectively. RESULTS: The data showed that quercetin has functions of inhibiting breeding of HeLa cells and inducing apoptosis of the cells. The total apoptosis rate and cell inhibition rate were positively proportional to concentration of quercetin used. The study suggests that quercetin has functions of inhibiting adhesion and migration and invasion of HeLa cells. The adhesive ratio, migration rate and invasiveness were negatively proportional to concentration of quercetin. The results demonstrated that with quercetin concentration increased from 20 to 80 micromol/l, cell inhibition rate increased from 37% to 83%; cell apoptosis rate increased from 18.71 +/- 2.61% to 70.00 +/- 4.05%; adhesive ratio decreased from 82% to 48%; migration rate decreased from 7.26 +/- 0.20 microm/hr to 3.78 +/- 0.64 microm/hr; invasiveness decreased from 124.33 +/- 1.53 piece to 90.67 +/- 2.08 piece (p < 0. 05). CONCLUSION: Quercetin can inhibit breeding of HeLa cells and induce apoptosis of the cells. Quercetin can inhibit adhesion and migration and invasion of HeLa cells. Quercetin may play an antimetastatic role.