Immobilization of beef liver catalase on eggshell membrane for fabrication of hydrogen peroxide biosensor

摘要

A simple and low-cost hydrogen peroxide biosensor was fabricated using a beef liver catalase-immobiliz edeggshell membrane and subsequently covered the surface of an oxygen electrode.The detection scheme was based on the increase in dissolved oxygen content upon exposure to hydrogen peroxide solution.The increase in oxygen level was then monitored and related to the hydrogen peroxide concentration.The effects of enzyme loading,dissolved oxygen content,pH,phosphate buffer concnetration and temperature on the biosensor have been studied in detail.The response and recovery times of the biosensor(t_95) were 1 min,respectively.The detertion limit based on 3sigma_b was 3 muM and the relative standard deviation of the response was 1.28% (n=10) for a solution containing 0.3 mM of H_2O_2.Th ehydrogen peroxide biosensor demosntrated a reasonable long and stable shelf-life.It has been successfullyapplied to the determination of hydrogenperoxide concnetration in some commercial samples.