Functional activity of L-carnitine transporters in human airway epithelial cells

摘要

Carnitine plays a physiologically important role in the beta-oxidation of fatty acids, facilitating the transport of long chain fatty acids across the inher mitochondrial membrane. Distribution of carnitine within the body tissues is mainly performed by novel organic cation transporter (OCTN) family, including the isoforms OCTN1 (SLC22A4) and OCTN2 (SLC22A5) expressed in human. We performed here a characterization of carnitine transport in human airway epithelial cells A549, Calu-3, NCl-H441, and BEAS-2B, by means of an integrated approach combining data of mRNA/protein expression with the kinetic and inhibition analyses of L-[H-3]carnitine transport. Carnitine uptake was strictly Na+-dependent in all cell models. In A549 and BEAS-2B cells, carnitine uptake was mediated by one high -affinity component (K-m <2 mu M) identifiable with OCTN2. In both these cell models, indeed, carnitine uptake was maximally inhibited by betaine and strongly reduced by SLC22A5/OCTN2 silencing. Conversely, Calu-3 and NCI-H441 exhibited both a high (K-m similar to 20 mu M) and a low affinity (K-m > 1 mM) transport component. While the high affinity component is identifiable with OCTN2, the low affinity uptake is mediated by ATB(0,+), a Na+, and Cl--coupled transport system for neutral and cationic amino acids, as demonstrated by the inhibition by leucine and arginine, as well as by SLC6A14/ATB(0,+) silencing. The presence of this transporter leads to a massive accumulation of carnitine inside the cells and may be of peculiar relevance in pathologic conditions of carnitine deficiency, such as those associated to OCTN2 defects. (C) 2015 Elsevier B.V. All rights reserved.