Biochemical effects of Cleome droserifolia on alloxan -induced diabetes in rats: Role of insulin, oxidative stress and inflammation

摘要

Oxidative stress associated with insulin -dependent diabetes mellitus is a risk for inflammatory disorders that induce renal dysfunction. Cleome droserifolia is a widely used for its antioxidant and antiinflammatory effects. This study aims to evaluate antihyperglycemic, antihyperlipidemic, antioxidant and anti inflammatory effects of methanolic Cleome droserifolia extract (MCD) against alloxan induced diabetes in male albino rats and explore possible effects of MCD on insulin and oxidative stress profile. Biochemical observations were further substantiated with histological examination of pancreas and kidney. Method: This study was carried on 30 male albino rats weighing 190±10, 90 days old, classified into 3 groups, control (NC), diabetic (DC) and treated diabetic group (MCD). Alloxan was given in a dose of 150 mg/kg intraperitoneally (IP). Result: Alloxan produced significant increase in serum glucose, Triglyceride (TG), Total cholesterol (TC), Low density lipoprotein cholesterol (LDL-C), urea and creatinine (Cr), renal reduced glutathione (GSH), renal nitrotyrosine, Renal TNF-α. On other hand, alloxan produced significant decrease in insulin levels and activity of renal catalase (CAT), renal glutathione peroxidase (GSH-Px) and renal superoxide oxide dismutase (SOD).Oral MCD (0.31 g/kg body weight of rats daily for 30 days)significantly ameliorated these effects. Conclusion: Treatment with MCD ameliorated DM and its related late consequences. Furthermore, it has antioxidant and antiinflammatory effects. Commonly, MCD is a way to surmount the diabetic state and it has antioxidant and antiinflammatory effects. It may be a promising adjuvant to anti diabetic therapy.