Tassani and Perilli [1] raised an interesting technical question in micro computed tomography (micro-CT) studies. We agree with them that characteristics of the scanned volume might also contribute to differences in obtained data, which should be also borne in mind when comparing different studies. Indeed, various micro-CT studies use a number of different approaches, various scanning resolutions, different segmentation and thresholding procedures and different shape and size of volumes of interest (VOI). In particular, the nominal resolution and voxel size varied significantly among studies, and the effects of that variability on bone rnicroarchitectural parameters have been recognised already [2].
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