Plantlet Regeneration of Potato Yam (Dioscorea bulbifera L.) through in Vitro Culture from Nodal Segments

摘要

Nodal vine segments from 6-week-old plants of Dioscorea bulbifera L. were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) and kinetin (Kn) together with a-naphthaleneacetic acid (NAA). Explants cultured in MS basal medium supplemented with 2.0 mg/1 Kn + 1.0 mg BAP + 0.5 mg/1 NAA showed highest number (8.5 ±0.51) of multiple shoots. When excised, shoots raised in vitro were inoculated onto half-strength MS basal media supplemented with 2.0 mg/1 NAA, where rooting was profuse (5.8 ± 0.39 roots per plantlet). Rooted shoots were transplanted to the greenhouse for hardening and; survival was 90% in field conditions without any visible morphological variation.