Effect of Enzymatic Treatment Using Proteases on Properties of Persistent Sodium Current in CA1 Pyramidal Neurons of Rat Hippocampus

摘要

We investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (I_(NaP)) in hippocampal CA1 pyramidal neurons. Properties of I_(NaP)were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase E (from Streptomyces griseus) or protease type XXIII (from Aspergillus oryzae). We have shown that kinetics of activation anddensity of I_(NaP) in neurons isolated with pronase E was unchanged. Enzymatic treatment with protease type XXIII did not alter I_(NaP) activation but resulted in a significant decrease in I_(NaP) density. Our data indicate that enzymatic treatment withpronase E for neuron isolation is preferable to investigate I_(NaP).