首页> 外文期刊>International journal of developmental neuroscience: the official journal of the International Society for Developmental Neuroscience >Postnatal growth hormone deficiency in growing rats causes marked decline in the activity of spinal cord acetylcholinesterase but not butyrylcholinesterase
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Postnatal growth hormone deficiency in growing rats causes marked decline in the activity of spinal cord acetylcholinesterase but not butyrylcholinesterase

机译:生长中大鼠的出生后生长激素缺乏会导致脊髓乙酰胆碱酯酶的活性明显下降,但不会引起丁酰胆碱酯酶的活性下降

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摘要

The effects of growth hormone (GH) deficiency on the developmental changes in the abundance and activity of cholinesterase enzymes were studied in the developing spinal cord (SC) of postnatal rats by measuring the specific activity of acetylcholinesterase (AChE), a marker for cholinergic neurons and their synaptic compartments, and butyrylcholinesterase (BuChE), a marker for glial cells and neurovascular cells. Specific activities of these two enzymes were measured in SC tissue of 21- and 90 day-old (P21, weaning age; P90, young adulthood) GH deficient spontaneous dwarf (SpDwf) mutant rats which lack anterior pituitary and circulating plasma GH, and were compared with SC tissue of normal age-matched control animals. Assays were carried out for AChE and BuChE activity in the presence of their specific chemical inhibitors, BW284C51 and iso-OMPA, respectively. Results revealed that mean AChE activity was markedly and significantly reduced [28% at P21, 49% at P90, (p< 0.01)] in the SC of GH deficient rats compared to age-matched controls. GH deficiency had a higher and more significant effect on AChE activity of the older (P90) rats than the younger ones (P21) ones. In contrast, BuChE activity in SC showed no significant changes in GH deficient rats at either of the two ages studied. Results imply that, in the absence of pituitary GH, the postnatal proliferation of cholinergic synapses in the rat SC, a CNS structure, where AChE activity is abundant, is markedly reduced during both the pre- and postweaning periods; more so in the postweaning than preweaning ages. In contrast, the absence of any effects on BuChE activity implies that GH does not affect the development of non-neuronal elements, e.g., glia, as much as the neuronal and synaptic compartments of the developing rat SC.
机译:通过测量乙酰胆碱酯酶(AChE)(胆碱能神经元的标志物)的比活性,在出生后大鼠的发育中的脊髓(SC)中研究了生长激素(GH)缺乏对胆碱酯酶的丰度和活性变化的影响。及其突触区室和丁酰胆碱酯酶(BuChE),它是神经胶质细胞和神经血管细胞的标志物。在21日龄和90日龄(P21,断奶年龄; P90,成年青年)的GH缺乏自发性矮化(SpDwf)突变大鼠中测量了这两种酶的比活性,这些大鼠缺乏垂体前叶和循环血浆GH,并且与正常年龄匹配的对照动物的SC组织相比。在分别存在其特定化学抑制剂BW284C51和iso-OMPA的情况下进行了AChE和BuChE活性的测定。结果显示,与年龄匹配的对照组相比,GH缺陷大鼠的SC的平均AChE活性显着降低[P21为28%,P90为49%(p <0.01)]。 GH缺乏对年长(P90)大鼠的AChE活性具有更高的影响,而对年幼(P21)大鼠的AChE活性具有更高的影响。相反,在所研究的两个年龄中的任一个年龄段,GH缺乏大鼠的SC中BuChE活性均无明显变化。结果表明,在没有垂体生长激素的情况下,大鼠SC中胆碱能突触的产后增殖,即断奶前和断奶后均明显降低了AChE活性丰富的CNS结构。在断奶后比断奶前的年龄更是如此。相反,对BuChE活性没有任何影响意味着GH不像发育中的大鼠SC的神经元和突触隔室那样影响非神经元例如胶质细胞的发育。

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