慢性阻塞性肺疾病大鼠肺组织中肺表面活性物质蛋白-C表达下降

摘要

Objective To study the role of surfactant protein C ( SP-C) in rat lung of chronic obstructive pulmonary disease (COPD).Methods Forty healthy conventional Sprague-Dawley (SD) rats were randomly divided into four groups , normal control group ( control group ) , smoke exposure group ( smoking group ) , lipopolysaccharide group (LPS group), smoke exposure +Lipopolysaccharide group (COPD group).The arterial partial pressure oxygen (PaO2) and arterial partial pressure of carbon dioxide pathological (PaCO2) were detected.The ultrastructure of lung tissue was observed by transmission electron microscope .Enzyme-linked immuno sorbent assay (ELISA) were performed to determine protein expression of SP-C in lung and bronchoalveolar lavage fluid ( BALF).RT-qPCR were performed to determine mRNA expression of SP-C in lung.Results Compared with control group , smoking group and LPS group, the PaO2 of COPD group was obviously lower , the PaCO2 of COPD group was obviously higher;the ultrastructure and histological analysis of lung tissues showed chronic inflammatory injury ; Compared with control group , the expression of SP-C protein in was reduced , as well as SP-C mRNA expression .Conclusions The expression of SP-C in lung of rats COPD model is down-regulated.SP-C may be involved in COPD .%目的 探讨肺表面活性物质蛋白-C(SP-C)在慢性阻塞性肺疾病(COPD)大鼠肺组织中的作用.方法 将大鼠随机分为对照组、香烟烟雾暴露组、脂多糖组和COPD组,每组10只.测定各组大鼠的PaO2和PaCO2的水平;透射电镜观察肺组织的细胞微观结构;ELISA检测支气管肺泡灌洗液(BALF)和肺组织SP-C蛋白;RT-qPCR检测肺组织SP-C mRNA的表达.结果 与其他组相比,COPD大鼠的PaO2最低,而PaCO2最高;肺泡Ⅱ型上皮细胞表面微绒毛明显减少(P<0.01);BALF和肺组织中SP-C蛋白表达下降(P<0.01);肺组织中的SP-C mRNA表达下降(P<0.01).结论 SP-C在COPD大鼠肺组织中表达下调,这种下调可能引起肺通气和肺换气功能障碍.