首页> 外文期刊>British journal of ophthalmology >Pericyte recruitment in human corneal angiogenesis: an ultrastructural study with clinicopathological correlation.
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Pericyte recruitment in human corneal angiogenesis: an ultrastructural study with clinicopathological correlation.

机译:人角膜血管生成中的周细胞募集:与临床病理相关的超微结构研究。

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BACKGROUND/AIM: During angiogenesis-that is, the outgrowth of new from pre-existing blood vessels, new capillaries undergo a period of "fine tuning" when vascular endothelial cells become apoptotic if sufficient supply of angiogenic factors is lacking. Morphologically, this period correlates with the absence of pericyte coverage of new vessels. Mature, pericyte covered vessels, in contrast, do not depend on elevated levels of angiogenic factors for survival. This study analyses whether, and if so when, pathological vessels in human corneal neovascularisation (CN) acquire pericyte coverage. This can be of importance for future angioregressive therapeutic strategies. METHODS: Vascularised human corneas obtained by keratoplasty were evaluated by electron microscopy for pericyte coverage of new vessels. These data were correlated with the duration of CN (mean 73 (SD 95) (range 0.5-360) months; n = 15). CN was secondary to keratitis, transplant rejection, aniridia, or trauma. RESULTS: Overall, 196 blood vessels were analysed ultrastructurally (72 (37%) capillaries, 122 (62%) venules, and two (1%) arterioles). Electron microscopically, 170 (87%) vessels were covered by pericytes and two (1%) in addition by smooth muscle cells. Pericyte recruitment increased with time, evolving between clinically noted onset of CN and keratoplasty. Already 2 weeks after onset of CN, more than 80% of new vessels were covered by pericytes. CONCLUSION: Pathological new vessels in human corneal angiogenesis are rapidly covered by pericytes. Therapeutic strategies aimed at regression of immature, not yet pericyte covered vessels by antagonising angiogenic factors should thus be most effective if applied very early in the course of corneal neovascularisation.
机译:背景/目的:在血管生成过程中(即新血管从原有血管中生长出来),如果血管内皮细胞缺乏足够的血管生成因子供应,新的毛细血管就会经历“微调”时期,此时血管内皮细胞会凋亡。从形态上讲,这一时期与新血管缺乏周细胞覆盖有关。相反,成熟的,被周细胞覆盖的血管并不依赖于升高的血管生成因子水平来生存。这项研究分析了人类角膜新血管形成(CN)中的病理血管是否以及是否获得了周细胞覆盖。这对于将来的血管退行性治疗策略可能很重要。方法:通过电子显微镜评估角膜移植术获得血管化的人角膜的新细胞周细胞覆盖率。这些数据与CN的持续时间相关(平均73(SD 95)(0.5-360)个月; n = 15)。 CN继发于角膜炎,移植排斥,无虹膜或外伤。结果:总体上,对196个血管进行了超微结构分析(72个(37%)毛细血管,122个(62%)小静脉和2个(1%)小动脉)。用电子显微镜观察,有170个血管(87%)被周细胞覆盖,另外两个血管(1%)被平滑肌细胞覆盖。周周募集随着时间增加,在临床上注意到的CN发作和角膜移植术之间发展。 CN发病后2周,超过80%的新血管被周细胞覆盖。结论:人角膜血管新生中的病理性新血管迅速被周细胞覆盖。因此,如果在角膜新生血管形成过程中尽早应用,旨在通过拮抗血管生成因子使未成熟,尚未被周细胞覆盖的血管消退的治疗策略应最为有效。

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