Liquid chromatographic determination of alogliptin in bulk and in its pharmaceutical preparation

摘要

In this work, a reversed-phase liquid chromatographic (RP-LC) method has been developed for the determination of alogliptin (ALG) based on isocratic elution using a mobile phase consisting of potassium dihydrogen phosphate buffer pH (4.6) - acetonitrile (20:80, v/v) at a flow rate of 1 mL min ~-1 with UV detection at 215 nm. Chromatographic separation was achieved on a Symmetry? cyanide column (150 mm × 4.6 mm, 5 μm). Linearity, accuracy and precision were found to be acceptable over the concentration range of 5-160 μg mL ~-1 for ALG in bulk. The optimized method was validated and proved to be specific, robust and accurate for the quality control of ALG in pharmaceutical preparations.