Kinetic and Thermodynamic Analysis of 9-Nitro-camptothecin Hydrolysis at Physiological pH in the Presence and Absence of Human Serum Albumin

摘要

Kinetic and thermodynamic analysis of the 9-nitrocamptothecin (9NC) hydrolysis reaction in the presence and absence of human serum albumin (HSA) in phosphate-buffered saline (PBS) of pH 7.4 was carried out by first derivative absorption spectroscopy. The thermodynamic parameters determined in these studies provided a mechanistic explanation toward the endothermic but yet thermodynamically favorable hydrolysis of 9NC at physiological temperature and pH. In the presence of HSA, the apparent rate constant of 9NC hydrolysis was 3-3.5 times higher than in 9NC solutions alone, whereas the apparent equilibrium constant of 9NC hydrolysis was found to increase at a higher extent in the presence of HSA than in PBS with increasing temperature, reaching almost complete hydrolysis of the 9NC to the 9NC-carboxylate at 315.15 K. Importantly, the E_a of the 9NC hydrolysis reaction in the presence of HSA was determined to be on average 17 kJ mol~(-1) lower than the E_a determined in plain PBS. Moreover, analysis of binding isotherms constructed for the HSA interaction with 9NC, using infinitely cooperative and independent binding models, demonstrated an incredibly higher binding constant for the 9NC-carboxylate form as compared to the very weak and concentration-dependent binding for the 9NC-lactone species at 310.15 K. Taken together, the preferential association of the carboxylate form with HSA and the lower E_a of 9NC hydrolysis in the presence of HSA provide a mechanistic explanation for the shift of lactone-carboxylate equilibria toward the carboxylate product under physiological conditions of pH and ionic strength.