CLONED PREPRO-HUMAN SERUM ALBUMIN GENE AND HUMAN SERUM ALBUMIN GENE, PLASMID CONTAINING THESE GENES,MICROORGANISM TRANSFORMED BY PLASMID,AND PRODUCTION OF PREPRO-HUMAN SERUM ALBUMIN BY USING SAID MICROORGANISM

摘要

PURPOSE: To produce a large amount of prepro-human serum albumin by the use of microorganisms transformed plasmids containing human serum albumin genes. ;CONSTITUTION: This cloned prepro-human serum albumin gene is obtained by grinding human liver cells in the presence of a ribonuclease-inactivating substance, removing solid cell pieces by a centrifugal separation method, etc., precipitating RNA from the obtained supernatant, subjecting the precipitated RNA to an affinity chromatography using oligo dT cellulose to separate human serum albumin mRNA from non-adenylated RNA, synthesizing a cDNA by the use of the mRNA as a template, inserting the cDNA into proper cloning vectors (preferably plasmid pBR 322), transforming microorganism cells with the cloning vectors, screening the obtained clones with e.g. a rat serum albumin cDNA, and subsequently culturing a positive clone in a proper culture medium.;COPYRIGHT: (C)1989,JPO