首页> 外文期刊>International journal of antimicrobial agents >Antimicrobial susceptibility of Neisseria gonorrhoeae isolates determined by the agar dilution, disk diffusion and Etest methods: comparison of results using GC agar and chocolate agar
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Antimicrobial susceptibility of Neisseria gonorrhoeae isolates determined by the agar dilution, disk diffusion and Etest methods: comparison of results using GC agar and chocolate agar

机译:通过琼脂稀释,圆盘扩散和Etest方法测定淋病奈瑟氏球菌分离株的抗菌药敏性:使用GC琼脂和巧克力琼脂的结果比较

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Although the use of GC agar for determining Neisseria gonorrhoeae antimicrobial susceptibilities is suggested by Clinical and Laboratory Standard Institute (CLSI) guidelines, chocolate agar is still used in some regions owing to its low cost and availability. To determine the differences in susceptibilities determined using GC and chocolate agars, 163 non-duplicate N. gonorrhoeae isolates were tested. Minimum inhibitory concentrations (MICs) and percent susceptibilities determined using the GC agar dilution method, respectively, were as follows: ceftriaxone, 0.004-0.125 mg/L, 100%; cefixime, 0.002 mg/L to > 32 mg/L, 98.2%; and ciprofloxacin, 0.002 mg/L to > 32 mg/L, 3.1%. Comparison of ceftriaxone MICs determined by the Etest using GC agar and chocolate agar showed that use of GC agar tended to result in lower MICs than GC agar dilution, whilst use of chocolate agar tended to result in higher MICs (concordance, 55.8% and 82.8%, respectively). Disk inhibition zones obtained using GC agar and chocolate agar (and their correlation coefficients) were, respectively: ceftriaxone, 35-55 mm and 25-50 mm (0.46); ciprofloxacin, 6-55 mm and 6-43 mm (0.84); and penicillin, 6-47 mm and 6-50 mm (0.93). Use of chocolate agar with the disk diffusion method for ceftriaxone was associated with a 5.5% false resistance rate. In summary, compared with GC agar, susceptibility testing using chocolate agar tends to yield higher MICs with the Etest and smaller disk inhibition zones with disk diffusion methods. Clinical microbiology laboratories should strictly adhere to CLSI recommendations by using GC agar instead of chocolate agar when performing susceptibility testing for N. gonorrhoeae.
机译:尽管临床和实验室标准协会(CLSI)指南建议使用GC琼脂确定淋病奈瑟菌的药敏性,但由于其价格低廉和实用性强,在某些地区仍使用巧克力琼脂。为了确定使用GC和巧克力琼脂测定的药敏性差异,测试了163个非重复性淋病奈瑟氏球菌。分别采用GC琼脂稀释法测定的最小抑菌浓度(MICs)和敏感性百分数如下:头孢曲松钠,0.004-0.125 mg / L,100%;头孢克肟,0.002 mg / L至> 32 mg / L,98.2%;环丙沙星为0.002 mg / L至> 32 mg / L,占3.1%。通过Etest使用GC琼脂和巧克力琼脂测定的头孢曲松钠MIC的比较表明,与GC琼脂稀释相比,使用GC琼脂倾向于导致较低的MIC,而使用巧克力琼脂则倾向于导致更高的MIC(一致性,分别为55.8%和82.8%) , 分别)。使用GC琼脂和巧克力琼脂获得的盘抑制区(及其相关系数)分别为:头孢曲松,35-55 mm和25-50 mm(0.46);环丙沙星,6-55毫米和6-43毫米(0.84);青霉素6-47毫米和6-50毫米(0.93)。碟形扩散法对头孢曲松的巧克力琼脂使用具有5.5%的错误抵抗率。总而言之,与GC琼脂相比,使用巧克力琼脂的磁化率测试倾向于通过Etest获得更高的MIC,而采用圆盘扩散方法则产生较小的圆盘抑制区。临床微生物实验室在进行淋病奈瑟氏球菌药敏试验时,应严格遵守CLSI建议,使用GC琼脂代替巧克力琼脂。

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