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首页> 外文期刊>International Biodeterioration & Biodegradation >Fungal diversity in ancient documents. A case study on the Archive of the University of Coimbra
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Fungal diversity in ancient documents. A case study on the Archive of the University of Coimbra

机译:古代文献中的真菌多样性。以科英布拉大学档案馆为例

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This multidisciplinary research combines knowledge in molecular biology with fungal morphology, aiming at the identification of infecting fungi from historical documents on the Archive of the University of Coimbra. The identification of infecting fungi on several bibliographic documents and support materials was based both on ribosomal DNA loci amplification and sequencing, and morphological identification, using macro- and microscopical traits. A high fungal diversity was found in all types of support: parchment, laid- paper and wood-pulp paper. Fourteen fungal genera were isolated, identified, and kept in culture. The most frequent were Cladosporium, Penicillium and Aspergillus, and other less frequent genera, such as Alternaria, Botrytis, Chaetomium, Chromelosporium, Epicoccum, Phlebiopsys and Toxicocladosporium were also present. Within these genera, 20 different species were identified, from which 15 were found only in a single support type. Cladosporium cladosporioides and Penicillium chrysogenum were the only species present in all support types.
机译:这项多学科研究将分子生物学知识与真菌形态学结合起来,旨在从科英布拉大学档案馆的历史文献中鉴定感染真菌。在几个书目文献和支持材料上对感染真菌的鉴定均基于核糖体DNA基因座的扩增和测序,以及利用宏观和微观特征进行的形态学鉴定。在所有类型的支撑物(羊皮纸,普通纸和木浆纸)上都发现了高度的真菌多样性。分离,鉴定并保存了十四个真菌属。最常见的是枝孢菌,青霉和曲霉,以及其他不常见的属,如链格孢属,葡萄孢属,Chaetomium,Chromelosporium,Epicoccum,Phlebiopsys和Toxicocladosporium。在这些属中,鉴定出20种不同的物种,其中仅在单一支持类型中发现了15种。在所有的支持物类型中,仅有的Cladosporium cladosporioides和Chicsogenum青霉。

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