The gene clusters encoding 18S+28S and 5S rRNA in the dog (Canis familiaris) have been localized by using GTG-banding and fluorescence in situ hybridization. The 18S+28S rDNA maps to chromosome regions 7q2.5→q2.7, 17q17, qter of a medium-sized, not yet numbered autosome, and Yql.2→q13. Our data show that there is one cluster of 5S rDNA in the dog, which maps to chromosome region 4q
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