A cell culture CO_2 incubator with continuous contamination control: part 2 - design features and evaluations

摘要

The dynamic nature of the cell culture CO_2 incubator presents a significant design challenge for the achievement of superior control of the purity of the incubator environment. Part 1 in American Biotechnology Laboratory (vol 20, no.1, pg. 16) reviewed the major concerns pertinent to the achievement of superior contamination control. Part 2 describes the development and validation of the model MCO-20 AIC (manufactured by SANYO Electric Biomedical Co., Ltd., Osaka, Japan; distributed by SANYO Scientific, Itasca, IL) approach to optimizing the cell culture environment through a process of Active Contamination Control~(TM). The cell culture CO_2 incubator incorporates a number of known and newly developed findings to create an envelope of activitiesto achieve continuous contamination control throughout the active processes involved. It employs an isolated, narrow-bandwidth ultraviolet (UV) light (Figure 1) to destroy air-borne contaminants in the incubator chamber, as well as water-borne organismsin the humidity water reservoir. Integrated with copper-enriched interior surfaces and components that inhibit the growth of organisms without suffering surface discoloration, the incubator offers an optimum cell culture environment that eliminates theneed for high heat decontamination, frequent chamber cleaning, and associated downtime.