Manipulating Rab GTPase activity in wheat to improve gluten quality for breadmaking

摘要

Sequence information of Rab GTPases in Arabidopsis, rice, Brachypodium and bread wheat was compiled by various means. These included online BLAST and string searches for genes, proteins, ESTs (expressed sequence tags), unigenes and GSSs (genome surveysequences). Sequences of 56 Rab genes from Arabidopsis, 44 from rice, 41 from Brachypodium, three from wheat and 26 wheat unigenes were collected. The sequence information was used to produce dendrograms from amino acid and nucleic acid sequences for comparison of Rab subfamilies in these species.A suitable candidate gene (Ta.54382) was chosen as the target for down-regulation. An RNAi construct was produced, targeting a region of the gene, and used to transform wheat explants.