Immobilization of aminoacylase by adsorption to tannin immobilized on aminohexyl cellulose

摘要

AbstractThe immobilization of aminoacylase (N‐acylamino acid amidohydrolase, EC 3.5.1.14) was investigated by using tannin immobilized on aminohexyl cellulose. The most active immobilized aminoacylase was obtained when aminoacylase was adsorbed to the immobilized tannin in a weak alkaline medium containing sodium chloride andn‐butanol at 37°C. The activity of the immobilized tannin–aminoacylase complex per unit volume was five times higher than that of the DEAE‐Sephadex–aminoacylase complex used for industrial production ofL‐amino acids in our plants. The half‐life of the immobilized tannin–aminoacylase complex was 20 days under continuous operation at a high concentration of substrate; on the contrary, that of the DEAE‐Sephadex‐aminoacylase