Tissue culture technology for rapid multiplication of Dendrocalamus giganteus Munro.

摘要

Micropropagation of Dendrocalamus giganteus through axillary bud culture is described. Multiple shoots were induced from in-vitro culture of nodal shoot segments containing axillary bud. Nodal segments produced axillary shoots in 2-3 weeks on Murashige and Skoog's (MS) medium supplemented with BAP (10-40 micro M). These shoots were excised from mother explants and subcultured on different concentrations of BAP in MS medium. Highest shoot multiplication rate of 5.44 fold was obtained on 20 micro M BAP medium. On MS medium supplemented with Kn only 3-4 fold shoot multiplication was obtained. The addition of BAP (10 micro M) in the kinetin (10 micro M) supplemented medium enhanced the shoot multiplication rate to 6.35 fold. 16-66% roots were formed on excised propagules of 3-5 shoots when transferred to MS medium supplemented with 15-25 micro M IBA. The maximum roots per propagule (8.66) and rooting percentage (90%) was obtained on MS medium supplemented with 25 micro M IBA+0.05 micro M BAP. These tissue culture raised plants were successfully hardened and acclimatized and transferred to field condition..