ZONA FREE HAMSTER OVA PENETRATION TEST: FERTILITY INDICATOR OF BUCKSPERMATOZOA

摘要

Being one of the most significant causes of fertilization failure, sperm functional impairment keeps till date a subject on which research has been carried out for a long time. Evaluation of the semen quality is the most important factor in estimationof fertility in domestic animals. Nowadays, acrosome reaction, hypo-osmotic swelling test, zona free hamster egg penetration test, cervical mucus penetration test and computer-assisted sperm analysis have been used for quality determination of semen (Avdatek et al., 2010). Traditionally, male fertility is defined by the evaluation of the number, motility, concentration and morphology of spermatozoa in the ejaculate (Vasan, 2011). As an essential principle, spermatozoal motility, concentration and number of morphologically normal spermatozoa must be above the minimum prescribed limits for successful conception and to determine fertility, functionalities of the spermatozoa play rather a determinant role than the number of spermatozoa do.