A new method for determination of trace phosphorous by energy transfer fluorescence quenching between Acridine orange and Rhodamine B has been developed.Effective energy transfer occurs between the two in presence of dodecyl benzene sodium sulfonate at lambda_(ex)/lambda_(ex)=450/575 nm and the fluorescence intensity of Rhodamine B increases sharply.Orthophosphate reacts with molybdate and forms molybdophosphate in hydrochloric acid medium,which diminishes the fluorescence intensity of Rhodamine B.The detection limit is up to 15.2 mu g l~(-1).The method is suitable for determination of phosphorous in the range,0.05~0.70mg l~(-1)and relative standard deviation is 1.75-3.12%.The recovery of phosphorous is 95.1-112.3%.The method has been applied to the determination of phosphorous in soil samples with satisfactory results.
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机译：建立了A啶橙与若丹明B之间能量转移荧光猝灭测定痕量磷的新方法，在十二烷基苯磺酸钠存在下，λ/（ex）/λ（ex）= 450 / 575 nm时，若丹明B的荧光强度急剧增加。正磷酸盐与钼酸盐反应，在盐酸介质中形成钼磷酸盐，降低了若丹明B的荧光强度，检出限高达15.2 mu gl〜（-1）。适用于0.05〜0.70mg l〜（-1）范围内的磷的测定，相对标准偏差为1.75-3.12％，磷的回收率为95.1-112.3％，该方法已用于磷的测定在土壤样品中获得满意的结果。
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